NaCl胁迫对骆驼蓬幼苗叶片多胺水平的影响

Effect of NaCl stress on polyamines level in Peganum harmala seedling leaves

研究了NaCl胁迫对骆驼蓬幼苗叶片多胺组分、多胺氧化酶(PAO)活性及H2O2和丙二醛(MDA)含量的影响。结果表明,不同浓度NaCl(0、50、100、200和400 mmol/L)胁迫8 d后骆驼蓬幼苗叶片游离态多胺含量随NaCl浓度增大明显下降,高氯酸不溶性结合态多胺下降较小,高氯酸可溶性结合态多胺无显著变化;不同形态多胺中腐胺(Put)含量随NaCl浓度提高显著降低,游离态和高氯酸可溶性结合态精胺(Spm)和亚精胺(Spd)含量先升后降,200 mmol/L NaCl时出现最大值,400 mmol/L时开始下降,但仍高于对照;高氯酸不溶性结合态Spm含量无显著变化而Spd则降低;游离态和细胞壁结合态PAO活性呈先升后降变化趋势;H2O2和MDA含量不断上升。

Three polyamines （Put, Spm and Spd） and their different forms （free, perchioric acid soluble and insoluble conjugated） together with polyamine oxidase （PAO） activities and the contents of H2O2 and malondialdehyde （MDA） in peganum harmala seedling leaves were investigated under NaCl stress. The results showed that with NaCl concentration （0, 50, 100, 200 and 400 mmol/L） increased, the total content of the free polyamines and the Put of three different forms of polyamines decreased dramatically, the insoluble conjugated ones decreased little and the soluble ones was significantly unchanged, the contents of free and soluble Spm and Spd were increased first and then decreased, while the insoluble Spm was not changed dramatically but Spd were decreased dramatically. The activities of free and cell wall-bound PAO were increased first and then decreased, and the contents of H2O2 and MDA were increased continuously.