补充辅酶Q10及递增负荷跑台运动训练对大鼠心肌和脑线粒体电子传递链酶复合体活性的影响

Effects of Exogenous(CoQ_(10))and Treadmill training on the Activities of Respiratory Chain Complexes of Rats Myocardium and Brain Mitochondria

目的:观察递增负荷跑台运动训练及补充CoQ_(10)对力竭运动大鼠心肌和脑线粒体呼吸链复合体Ⅰ、Ⅱ和Ⅲ活性的影响。方法:36只健康雄性、Wister大鼠随机分为安静对照组、补充CoQ_(10)组、训练组和训练结合补充CoQ_(10)组。差速离心提取心肌和脑线粒体;分光光度法测定线粒体呼吸链酶复合体(Ⅰ～Ⅲ)活性。结果:1)安静状态下补充CoQ_(10) 7周,与安静对照组相比较,力竭运动后即刻心肌线粒体呼吸链CⅡ活性增强(P<0.01),CⅠ和CⅢ活性减弱(P<0.01);脑线粒体呼吸链CⅡ活性显著性降低(P<0.01)。2)递增负荷运动训练7周,与安静对照组相比较,力竭运动后即刻心肌线粒体呼吸链CⅠ和CⅡ活性减弱(P<0.01)、CⅢ活性增强(P<0.05)。3)递增负荷训练结合补充CoQ_(10) 7周,与安静对照组相比较,力竭运动后即刻心肌线粒体呼吸链CⅠ和CⅡ活性减弱(P<0.01),脑线粒体呼吸链CⅡ活性显著性下降(P<0.01)。4)递增负荷训练结合补充CoQ_(10)与单纯补充CoQ_(10)组相比,力竭运动后即刻心肌线粒体呼吸链CⅡ活性减弱(P<0.01)、CⅢ活性增强(P<0.01);脑线粒体呼吸链CⅡ活性显著性下降(P<0.01)。5)递增负荷训练结合补充CoQ_(10)与单纯运动训练组相比,力蝎运动后即刻心肌线粒体呼吸链CⅡ和CⅢ活性减弱(P<0.01);脑线粒体呼吸链CⅡ活性显著性下降(P<0.01)。结论:外源性补充CoQ_(10)对大鼠体重增长无负面影响,运动训练可以降低大鼠体重增长幅度。单纯补充CoQ_(10)可增强力竭运动后即刻大鼠心肌线粒体呼吸链CⅡ活性;降低脑线粒体呼吸链CⅡ活性;对力竭运动时间无影响。递增负荷训练可增强力竭运动后即刻大鼠心肌线粒体呼吸链CⅢ活性;对脑线粒体呼吸链CⅠ、CⅡ和CⅢ活性无明显影响;运动能力得到提高。递增负荷训练结合补充CoQ_(10)可维持力竭运动后即刻大鼠心肌线粒体呼吸链CⅢ活性不降低;脑线粒体呼吸链CⅡ活性显著性下降、CⅠ和CⅢ活性无显著性变化;运动能力得到提高;运动训练与补充CoQ_(10)对改善心肌和脑线粒体呼吸链CⅠ、CⅡ和CⅢ活性及运动能力方面无协同作用。训练组及训练结合补充CoQ_(10)组大鼠力竭运动时间的延长可能还与体重增长较慢及其他机制有关。

The purpose of this study is to examine the effects of exogenous coenzyme Q10 （CoQ10） and training on the activities of respiratory chain complexes of rats myocardium and brain mitochondria and try to find a way to and nutrition supplement. 36 healthy male improve mitochondrial function by exercise-training two-month-old Wister rats were randomly divided into 4 groups as static control （ NC）, supplementation of CoQ10 （ QC）, exercise-training （NE） and training with supplementation of CoQ10 （QE）. Mitochondria were isolated in different speed centrifugal way. Spectrophotometric analysis was applied to evaluate RCC （ Ⅰ-Ⅲ ） activities in myocardium mitochondria. Results： 1. Compared with NC, rats from QC showed a significant increase of myocardium RCC activities in C Ⅱ （P〈0.01）, a significant decrease in C Ⅰ and C Ⅲ （P〈0.01） ;a significant decrease of brain RCC activities in C Ⅱ （P〈0.01） ;2. Compared with NC, rats from NE represented a significant decrease of myocardium RCC activities in C Ⅰ and C Ⅱ （P〈0. 01） and a marked raise of cm （P〈0. 05）, 3. Compared with NC, training with supplementation of CoQ10 led to a significant decrease of myocardium RCC activities in C Ⅰ and C Ⅱ （P〈0.01）, a significant decrease of brain RCC activities in C Ⅱ （P 〈0.01）. 4. Compared with QC, rats from QE showed a distinct decrease of myocardium RCC activities in CⅡ （P〈0.01） and a significant increase of CⅢ （P〈0.01）, a distinct decrease of brain RCC activities in C Ⅱ. 5. Compared with NC, rats from QE represented a significant decrease of myocardium RCC activities in C Ⅱ and C Ⅲ （P〈0.01）, a significant decrease of brain RCC activities in CⅡ （P〈0.01. conclusion.1, there was no effect of CoQ10 supplementation on rats＇ weight, however, exercise-training could reduce the rate of mice＇s weight increasing. 2. CoQ10 supplementation to rats could improve activities of C Ⅱ in mitochondria from myocardium, could decrease the activity of C Ⅱ in mitochondria from brain, but had no effect on the time of rats＇ running to exhaustion. 3. Treadmill training could improve activities of C Ⅲ in mitochondria from myocardium and could no marked differences in C Ⅰ, C Ⅱ and C Ⅲ in mitochondria from brain and could improve rats＇ sport ability. 4. Training with supplementation of CoQ10 could maintain activities of C Ⅲ in mitochondria from myocardium and could decrease the activities of C Ⅱ （P〈0.01）, no marked different in C Ⅰ and C Ⅲ in mitochondria from brain and postpone the time of occurrence of exercise-fatigue, but there was no better function of cooperation between supplementation of CoQ10 and training to improve RCC （ Ⅰ-Ⅲ） activities from mitochondria in myocardium and brain. 5. The prolonged running time to exhaustion of rats from NE and QE might relate to their less weight increasing and other mechanism,