摘要
利用L-半胱氨酸对血红蛋白模拟酶催化体系的抑制作用，建立了酶抑制动力学光度法测定L-半胱氨酸的新方法。研究了该抑制反应的最佳实验条件及动力学行为，测定的线性范围为2．2×10^-7～8．8×10^-6mol／L，检出限为5．4×10^-8mol／L。对浓度为7．8×10^-6mol／L的L-半胱氨酸进行11次平行测定，其相对标准偏差为4．1％。该法已用于明胶中L-半胱氨酸的测定。
A high sensitive and simple spectrophotometric method for the determination of L-cysteine based on the inhibition of hemoglobin for the oxidation of H2O2 with methylene blue was developed. The percentage inhibition （1%） of system is calculated under the optimal experimental conditions. The calibration curve is linear in the range of 2.2 ×10^-7～ 8.8×10^-6 mol/L with the detection limit of 5.4 × 10^-5 mol/L, The relative standard deviation of this method is 4.1% at 7.8 ×10^-6 mol/L for 11 determinations. This method can be used for the determination of L-cysteine in gelatin with satisfactory results.
出处
《分析试验室》
CAS
CSCD
北大核心
2008年第1期38-41,共4页
Chinese Journal of Analysis Laboratory
基金
河南省教育厅自然科学基金资助项目(2007150048)
