高效液相色谱——荧光检测器测定蜂王浆中磺胺类药物残留的研究

Determination of sulfonamide residues in royal jelly by HPLC-fluorescence detector

本文采用液液萃取进行样品前处理,柱前衍生后用带荧光检测器的液相色谱仪检测蜂王浆中的5种磺胺类药物残留,具有方法简单,快速,准确,试剂用量少,检测成本低的特点。该方法的检测限为1μg/kg,能满足目前蜂王浆出口中磺胺类药物残留允许限量的要求。

The purpose of this paper using liquid-phase extraction as pre-treatment, five sulfonamide residues in royal jelly were determined by pre-column derivatization HPLC with fluorescence detector. The proposed method, with less reagent and cost, was simple, rapid and accurate. The detection limit was 1μg/kg, which could meet the need of the residue limit for sulfonamide in royal jelly of exportation.