超短波并川芎嗪雾化吸入疗法对支气管哮喘细胞因子失衡的调节作用

The role of ultrashort wave diathermy and ligustrazine fog inhalation in regulation of cytokine disequilibrium in asthmatic patients

目的探讨超短波加川芎嗪雾化吸入疗法对支气管哮喘细胞因子失衡的调节作用。方法选取轻、中度支气管哮喘患者70例，随机分为超短波加川芎嗪雾化吸入治疗组（超短波组）36例和单纯川芎嗪雾化吸入治疗组（吸入疗法组）34例，疗程为2周。另选本院无吸烟史的健康体检者30例作为健康对照组。所有患者于入院第1天治疗前及治疗2周后进行症状体征评分，记录无症状天数、β2受体激动剂吸入量，测定肺功能指标，包括1秒钟用力呼气容积（FEV1）、最大呼气流量（PEF）。3组均抽取静脉血，采用双抗体夹心酶联免疫吸附测定法检测IL-4和IFN-γ水平。结果与健康对照组比较，哮喘患者血清IL-4水平升高、IFN-γ水平降低，差异均有统计学意义（P〈0．05）。2组患者经治疗后，临床症状评分下降，FEV1、PEF升高，血清IL-4水平、IL-4／IFN-γ比值降低，与治疗前比较，差异有统计学意义（P〈0．05）；且超短波组上述各指标变化更明显，与吸入疗法组比较，差异有统计学意义（P〈0．05）。2组患者IFN-γ水平虽有升高，但与治疗前比较，差异无统计学意义（P〉0．05）。结论超短波加川芎嗪雾化吸入治疗支气管哮喘有较好的临床疗效，其作用机制可能与调节失衡的TH1／TH2平衡有关。

Objective To explore the regulating effects of ultrashort wave diathermy and ligustrazine fog inhalation on cytokine disequilibrium in asthmatic patients. Methods Seventy patients with mild and moderate asthmatic were recruited and randomly divided into two groups. Group Ⅰ （ n = 36） was given ultrashort wave diathermy and ligustrazine fog inhalation, group Ⅱ （ n = 34） underwent ligustrazine fog inhalation only. The interventions lasted for two weeks. Thirty healthy nonsmoking persons were recruited and served as controls. Before and after 2 weeks of treatment, the clinical manifestations including the symptoms and signs, the number of non - symptomatic day and the amount of β2 receptor activator inhalated were recorded and scored, the index of lung function including the forced expiratory volume at one second （FEV1） and the peak expiratory flow （PEF） was assessed, the serum concentration of IL-4 and IFN-γ, were detected by ELISA with all the patients and the controls. Results It was shown that the concentration of IL-4 were higher and the level of IFN-γ, were lower in the asthmatic patients than those in the controls （P 〈0.05）. After treatment, the symptom scores were decreased, FEV1 and PEF increased, the serum concentration of IL-4 and the ratio of IL-4/IFN-γ, decreased in the two treatment groups, with the group Ⅰ changed to a significantly larger extent （ P 〈 0. 05 ）. However, although the serum concentration of IFN-γ was increased after treatment, the change did not have statistical significance （ P 〉 0. 05 ）. Conclusion Ultrashort wave diathermy and ligustrazine fog inhalation treatment were efficacious for treating the asthmatic patients, which might be associated with the inhibition of the inflammation via regulating TH1/TH2 balance.