高效液相色谱法测定鸡肉中己烯雌酚

HPLC Determination of Diethylstibestrol in Chicken

采用高效液相色谱法（HPLC）测定鸡肉中己烯雌酚残留量，将碎鸡肉用甲醇超声提取，合并提取液浓缩近干，用甲醇溶解残渣，所得溶液用于HPLC测定。Symmetry C18用作色谱柱，甲醇与0．0128mol·L^-1磷酸二氢钠溶液以63比37（体积比）混合后作为流动相，流速为1．0mL·min^-1。在230nm波长处，用二极管阵列检测器检测。对鸡肉样品作了分析，测定结果的相对标准偏差（n=5）均小于10％，回收试验给出回收率在87．5％～92．3％之间。测得方法的检出限（S／N=3）为20μg·kg^-1。

High performance liquid chromatography （HPLC） was applied to the determination of residual diethylstibestrol in chicken as food. Smashed and homogenized chicken sample was extracted ultrasonically with methanol for several times as directed in the procedure. The extracts were combined and evaporated to near dryness. The residue was taken up with 1 mL of methanol and the solution was used for HPLC determination. Symmetry C18 was used as the chromatographic column and a mixture of methanol and 0. 012 8 mol·L^-1 NaH2 PO4 solution mixed in the ratio of 63 to 37 （ by volume）, adjusted to pH 5, was used as the mobile phase at a flow-rate of 1.0 mL·min^-1. UV-detection at 230 nm with array photodiode was used in the determination. Detection limit （S/N=3） was found to be 20μg·kg^-1. Sample of chicken was analyzed by this method and values of RSDrs （n= 5） and recovery were found to be less than 10% and in the range of 87.5% to 92.3% respectively.