多波长LED阵列诱导荧光光谱在多组分分析中的应用研究

Study on Application of Multi-Wavelength LED Array Induced Fluorescence Spectrum in Multicomponent Analysis

荧光光谱技术是对水中的溶解有机物、油类污染物,以及各种浮游植物生物量识别与测量的有效手段之一。构建了多波长LED阵列(450～610nm)诱导荧光光谱测量系统,讨论了系统的原理和利用此系统进行多组分分析的方法。在实验室应用该系统获取了多组分混合荧光物质溶液的三维荧光光谱,并采用平行因子算法分析了多组分三维荧光光谱数据,结果显示得出的多组分浓度值与实际浓度的相关性大于98%。表明基于多波长LED阵列诱导荧光光谱测量系统在多组分分析中具有很好的应用前景。

The technique of fluorescence spectrum is one of the efficient methods for monitoring and identifying dissolved organic matter, oil pollutants and biomass of phytoplankton in water. A new kind of fluorescence spectrum system with a multi-wavelength LED array as the exciting light source is introduced. The principle of the system and the method for multi-component analysis are discussed. The excitation-emission matrix （EEM） fluorescence spectra of samples mixed with different concentration fluorescent dyes were measured with this system in the authors’ laboratory, which were analyzed using parallel factor （PARAFAC） algorithm. The results indicate that the correlation coefficient of the resolved concentration and actual ones is up to 98%, which shows the potential application of the fluorescence spectrum system in multi-component analysis.