焦炉工外周血淋巴细胞热应激蛋白的表达与细胞DNA损伤程度

Relationship between the expression of heat shock protein and genetic damage in peripheral blood of workers exposed to coke oven emissions

目的探讨焦炉工外周血淋巴细胞热应激蛋白90、60及27（HSP90、HSP60和HSP27）的表达水平与外周血淋巴细胞DNA损伤之间的关系。方法选取某焦化厂焦炉作业工人288名为接触组，根据作业环境监测结果和工作岗位分为高剂量组（炉顶及炉侧工）172人，低剂量组（炉底及辅助工）116人；同时选取距该焦化厂2km外、无有毒作业接触史的某医院医护人员和管理人员38名为对照组。取受试者的肘静脉血，应用Western blot法测定外周血淋巴细胞的HSP90、HSP60和HSP27水平。用彗星试验测定外周血淋巴细胞DNA的损伤程度。结果高剂量组焦炉工淋巴细胞HSP90水平（0．24±0．32）明显高于低剂量组（0．12±0．30）和对照组（0．06±0．33），差异有统计学意义（P〈0．05）；各组HSP60、HSP27表达水平比较，差异均无统计学意义（P〉0．05）。高剂量组、低剂量组及对照组间彗星的尾长、Olive尾矩、尾惯量、尾／头（长）总体均呈现高剂量组〉低剂量组〉对照组的趋势，这4项指标分别在各组间的差异均有统计学意义（P〈0．05）。随接触剂量增高，DNA损伤程度逐渐加重，3组人群的DNA损伤分级的相关系数为-0．345，差异有统计学意义（P〈0．01）。结论焦炉工外周血淋巴细胞HSP90表达水平及DNA损伤程度随多环芳烃接触剂量的增高而增高。

Objective To explore the relationship between the expression of heat shock protein 90,60 and 27（HSP90, HSP60 and HSP27 ） and genetic damage in peripheral blood of workers exposed to coke oven emissions. Methods 288 coke oven workers in a steel factory were divided into the high-dose group and the lowdose group on the basis of environment monitoring result and work place. There were 172 men in high-dose group （workers who worked at the oven top and oven side） and 116 men in low-dose group （workers who worked at the oven bottom and others who were engaged to aided work）. 38 workers unexposed occupationally to carcinogenic substances were selected as the control group,who were employed in medical therapy unit nearby 2 kilometers from the steel factory. Their general information,history of personal and occupational ex-posure, and the work environment were investigated. Blood samples were collected immediately after a shift at the end of a working day from 288 coke oven workers and 38 control workers. Levels of HSP90, HSP60 and HSP27 in peripheral blood lymphocytes were measured by Western blot, and the degree of DNA damage was detected by the comet assay. Results Levels of HSP90 in peripheral blood lymphocytes in three groups were 0.24±0.32,0.12±0.30 and 0.06±0.33 respectively. They increased significantly compared with that of the control. But levels of HSP60 and HSP27 were not significantly different among those groups. Compared with the control group ,there was significant difference in tail length,olive tail moment et al of SCGE （G±SG）of occupational exposure workers. High-dose group〉low-dose group〉control group （P〈0.05）. The degree of DNA damage increased with the rise of exposure BaP dose（Spearman r=-0.345,P〈0.01 ）. Conclusion Levels of HSP90 in peripheral blood lymphocytes and the degree of DNA damage increase with the rise of exposure polycyclic aromatic hydrocarbons（PAHs） dose.