荧光定量PCR检测HBV-DNA的临床价值

Clinical Value of Quantitative Detection of HBV-DNA with FQ-PCR.

目的:探讨荧光定量PCR(FQ-PCR)检测乙型肝炎病毒(HBV)DNA的临床价值。方法:用FQ-PCR检测860份血清标本中HBV-DNA含量,同时用ELISA法检测乙肝血清学指标,即两对半(HBsAg、HBsAb、HBeAg、HBeAb和HBcAb)。结果:HBsAg(+)HBeAg(+)HBcAb(+)组(大三阳),HBV-DNA阳性率为97.00%,HBV-DNA平均含量为7.34±0.73;HBsAg(+)HBeAb(+)HBcAb(+)组(小三阳),HBV-DNA阳性率37.93%,HBV-DNA平均含量为4.36±1.34;HBsAg(+)HBcAb(+)组,HBV-DNA阳性率为61.11%,HBV-DNA平均含量为4.82±1.47;HBsAg(+)HBeAg(+)组,HBV-DNA阳性率为100%,HBV-DNA平均含量为7.03±0.83;HBsAb(+)HBeAb(+)HBc(+)模式HBV-DNA也有低水平复制。结论:荧光定量PCR在乙肝的诊断、判断传染性强弱及抗病毒治疗效果方面具有较大的价值。

Objectives： To explore the clinical value of detecting hepatitis B virus DNA by fluorescence quantitative polymerase chain reaction （FQ-PCR） . Methods： FQ-RCR was used to detect the concentration of HBV-DNA in 860 serum samples, and ELISA was used to detect hepatitis B virus serological makers （HBV-M） . Results： The HBV-DNA positive rate was 97.00%, and the average copies were 7.34± 0.73/ml in the group [HBsAg （ ＋ ） HBeAg （ ＋ ） HBcAb （ ＋ ）] . The HBV-DNA positive rate was 37.93%, and the average copies were 4.36± 1.34/ml in the group [HBsAg （ ＋ ） HBeAb （ ＋ ） HBcAb （ ＋ ）] . The HBV-DNA positive rate was 61.11%, average copies were 4.82±1.47/ml in the group [HBsAg （ ＋ ） HBcAb （ ＋ ）] . The HBV-DNA positive rate was 100%, average copies were 7.03 ± 0.83/ml in the group [ HBsAg （ ＋ ） HBeAg （ ＋ ） ], and low-level HBV-DNA copy was measured in the group [HBsAb （ ＋ ） HBeAb （ ＋ ） HBcAb （ ＋ ） ] . Conclusions： FQ-PCR is significant for chronic hepatitis B diagnoses, determination of infectivity and therapy effect.