复智散对阿尔茨海默病模型小鼠认知功能及脑内胆碱能系统的影响

Effect of Fuzhisan(FZS) on cognitive ability and cholinergic system of Alzheimer′s disease (AD) model mice

目的观察复智散(FZS)对阿尔茨海默病(AD)模型小鼠认知功能及脑内胆碱能系统的影响。方法采用β-淀粉样肽(Aβ25-35)小鼠侧脑室注射制作AD小鼠模型,应用水迷宫和小鼠穿梭程序自动控制仪检测小鼠的认知功能,采用比色法测定小鼠脑内胆碱乙酰基转移酶(ChAT)和乙酰胆碱脂酶(AchE)活性,采用碱性羟胺法测定乙酰胆碱(Ach)含量。结果FZS能明显改善AD小鼠的认知功能,且对脑内胆碱能系统有明显的调节作用,不仅能够降低AchE活性,而且能够增强ChAT活性,增加脑内Ach的含量。结论FZS可通过对脑内胆碱能系统的调节来改善其认知功能,从而达到治疗AD的效果。

Objective To observe the effect of FZS on cognitive ability and cholinergic system in the brain of Alzheimer＇s disease （AD） model mice induced by intraventricular Aβ25-35 injection. Methods The AD model mice was established by injected Aβ25-35 to lateral ventricle. The cognitive ability of AD model was detected by water amaze test and shuttle box test. The activity of acetylcholinesterase（AchE） and choline acetyhransferase（CHAT） in the brains were examined by colorimetric method. The acetylcholine（Ach） content was examined by alk hydroxylamine colorimetric method. Results FZS effectively improved cognitive performance, including learning and memory ability of the AD model mice, and not only degraded AchE activity, but also enhanced ChAT activity and Ach content in the AD model mice brain. Conclusions FZS can effectively improve cognitive performance by regulating cholinergic system function in the brain and cure AD.