摘要
目的探讨ghrelin基因多态性与2型糖尿病之间的关系。方法进行ghrelin基因C408A和G346A的多态性分析,同时进行生化指标和临床参数的检测。结果正常对照组(NGT组)C408A基因型CC、CA、AA的基因型频率分别为75.4%、24.0%及0.6%。2型糖尿病组(DM组)中CC、CA及AA的基因型频率分别为70.8%、28.2%及1.0%。NGT组CC基因型者总胆固醇水平明显高于CA+AA基因型者(P<0.05);DM组CC基因型者血尿酸水平明显高于CA+AA基因型者(P<0.05)。在所有的受试者中未发现G346A多态性存在。结论Ghrelin基因C408A分布与等位基因频率没有明显的差异;本组人群中未发现ghrelinG346A多态性存在;C408A多态性与总胆固醇和尿酸水平相关。
Objective To study the relationship between ghrelin gene polymorphisms and type 2 diabetes. Methods All subjects were genotyped for these two single nucleotide polymorphisms （C408A, G346A ） of ghrelin by PCR-RFLP assay. Anthropometric variables, blood glucose, insulin, lipid, uric acid and other clinic parameters were measured. Results In control group, the genotype frequencies are as follows ： CC 75.4% （ n = 251 ） , CA 24. 0% （n =80） , and AA 0. 6% （n =2）. In type 2 diabetes group, the genotype frequencies are as follows： CC 70. 8% （n = 143） , CA 28.2% （n =57） , and AA 1.0% （n =2）. There was no significant differences of the genotype frequencies in two groups. In control group, serum total cholesterol in subjects carrying CC genotype was higher than that of subjects without CC genotype （ P 〈 0. 05 ） ; in type 2 diabetes groups, serum uric acid in subjects carrying CC genotype was higher than that of subjects without CC genotype （ P 〈 0. 05 ）. There was no G346A variants existed in our study subjects. Conclusion The allele frequencies and genotype distribution of the C408A was not different between control and type 2 diabetes groups; G346A polymorphism was not found in control and type 2 diabetes groups. C408A variants is related with total cholesterol and uric acid.
出处
《基础医学与临床》
CSCD
北大核心
2008年第1期44-47,共4页
Basic and Clinical Medicine
基金
首都医学发展科研基金(2002-1017)
