1-磷酸鞘氨醇延长豚鼠心室肌细胞动作电位及抑制电压依赖性钾电流

Sphingosine 1-phosphate prolongs action potential duration and inhibits voltage-dependent potassium current in guinea-pig ventricular myocytes

目的研究1-磷酸鞘氨醇(S1P)对豚鼠心室肌细胞动作电位(AP)及电压依赖性钾电流(KV)的作用及其机制。方法利用Langendorff离体心脏逆向灌流法分离豚鼠心室肌细胞,采用全细胞膜片钳技术记录心室肌细胞AP和KV。结果S1P可延长动作电位复极50%时程(APD50)和动作电位复极90%时程(APD90),降低KV,其作用可被百日咳毒素(PTX)和卡弗他丁C(Calphostin C)阻断。结论S1P可能通过PKC途径抑制KV及延长AP时程。

Objective To investigate the effect and mechanism of sphingosine 1-phosphate （S1 P） on action potential （AP） and voltage-dependent potassium current （Kv）in isolated guinea pig ventrieular myocytes. Methods The yentricular myocytes were isolated by using Langend patch-clamp recording technique. Results S1P orff perfusion method. The AP and Kv were recorded by whole cell prolonged the 50% and 90% action potential duration （APDso and APDgo） and decreased Kv which were blocked by pertussis toxin （FIX） or Calphostin C. Conclusion SIP decreased Kv, in a PKC pathway and prolonged action potential duration in guinea-pig ventrieular myoeytes.