摘要
目的研究卵圆细胞在肝损伤中的修复作用,并对其可能的作用机制进行初步探讨。方法清洁级SD大鼠50只随机分为正常对照组(20只)和模型组(30只),两组均于实验第5周取肝组织进行常规病理、透射电镜,免疫组化检测波形蛋白(vimentin)和增殖细胞核抗原(PCNA)蛋白表达,半定量RT-PCR方法检测Notch1、RBP-Jκ、HES1mRNA表达。结果正常对照组大鼠肝组织学形态正常,偶见vimentin和PCNA阳性细胞,Notch1、RBP-Jκ、HES1mRNA低表达;模型组主要于汇管区发现大量卵圆细胞,电镜下可见卵圆细胞的特征性表现张力微丝和桥粒连接,免疫组化示vimentin和PCNA阳性细胞明显增加(P<0.01),Notch1、RBP-Jκ、HES1 mRNA表达显著上调(P<0.01)。结论卵圆细胞在肝损伤修复中可能发挥重要作用,其作用机制与Notch家族有关。
Objective To explore the role of oval cells in hepatic injure and to study its mechanism. Methods Fifty male SD rats were randomly divided into control group（20 cases） and model group （30 cases）. Hepatic pathologic changes were observed through optical microscope and electron microscope. Vimentin and PCNA were detected by immunohistochemistry, Notchl .,RBP-JK, HES1 mRNA were detected by semi-quantitative RT-PCR method. Results The histological morphology was normal in the control group, the positive cells of vimentin and PCNA were found on occasion, the expression of Notchl .,RBP-JK,HES1 mRNA was low. In the model group,the oval cells were discovered in portal duct regions massively. The tonofilaments and desmosome juntion which were the distinctive structure of oval cells were observed by electron microscopy. The positive cells of vimentin and PCNA were increased （P 〈 0.01 ）, the expression of Notchl 、RBP-JK、HES1 mRNA were increased （P 〈 0. 01 ）. Conclusion The oval cells play an important role in repair of hepatic injury, the mechanism might be explained by to Notch family.
出处
《基础医学与临床》
CSCD
北大核心
2008年第1期61-65,共5页
Basic and Clinical Medicine
