米非司酮基因诱导调控系统

Advancement in Mifepristone -inducible Regulatory System

在基因表达和基因治疗研究中,需要目的基因在特定的时间以适当的水平实现其表达,目的基因过度表达或不适当的表达将影响实验结果,在疾病治疗中甚至会产生致命的副作用,实现对目的基因的表达时间和表达水平的精确调控是一个非常关键的问题。目前生物学家们已构建了多种新型的基因表达调控系统,其中米非司酮诱导调控系统具有诱导效率高,背景表达低,安全性高等诸多优点,是基因调控研究中的重要进展,也是目前最有应用前景的调控系统之一。就其结构设计和应用研究方面的进展作一简要综述。

It is important that the the expression level and timing time of a target gene is regulated and controlled in the study of gene therapy and gene function. Constitutive or inappropriate expression of the genes with traditional expression systems may interfere with the effects of the gene therapy, even may lead to lethal side effects. Inducible expression systems can be used to regulate the expression level of genes of interest in appropriate time according to the aim of the experiments. Up to date, several inducible expression systems have been established and become the potent tools for gene therapy and studying the function of genes of interest. One of the most promising regulatory systems is the mifepristone-regulated system, which has much lower basal transcriptional activity and high inducibility. Recent developments in system design and application was reviewed.