适配子筛选技术

Methodology for Aptamer Selection

适配子的筛选技术近年来不断改良与发展。结合核酸与游离核酸的分离技术的改良,使适配子筛选效率得到提高;无引物结合部位适配子的筛选技术的建立和短寡核苷酸文库的应用,使适配子的应用范围更加广泛;适配子筛选技术在功能上的拓展,满足了更多的研究要求。一系列新方法的建立和应用,使适配子在基础研究和临床应用方面更具潜在价值。

The methods for aptamer selection have efficiency has been increased due to the improvement been modified and developed in recent years. Selection in separation of bound and unbound nucleic acids; applicability of aptamers has been improved due to the development of primer-free SELEX and the utilization of short oligonucleotide library; more research requirements have been satisfied due to the functional modifications of aptamer selection methods. All the methodological advances enhance the potential value of aptamers in basic researches and clinical applications.