尾加压素Ⅱ与GPR14 mRNA在子痫前期患者胎盘组织中的表达及意义

Expression of UⅡ and GPR14 mRNA in placenta of patients with preeclampsia.

目的研究子痫前期患者胎盘组织尾加压素Ⅱ(UⅡ)及G蛋白偶联受体14(GPR14)mRNA的表达变化及其与子痫前期发病的关系。方法采用RT-PCR方法对25例子痫前期(子痫前期组,其中轻度15例,重度10例))患者和20例正常足月孕妇(正常妊娠组)和胎盘组织中UⅡ和GPR14 mRNA表达水平进行检测。结果胎盘组织UⅡmRNA表达水平在轻度子痫前期组,重度子痫前期组均明显高于正常妊娠组,差异有统计学意义(P均<0.05)。重度子痫前期组孕妇胎盘GPR14mRNA表达水平明显高于对照组,差异有统计学意义(P<0.05)。结论子痫前期患者胎盘UⅡ及其受体基因表达升高,可能在子痫前期胎盘缺血缺氧动脉粥样硬化的发生中发挥重要作用。

Objective：To study the mRNA expression of urotensinⅡ（UⅡ） and G-protein coupled receptor 14（GPR14） in placenta of preeclampsia patients and their relation to the pathogenesis of preeclampsia.Methods：Expression of UⅡ and GPR14 mRNA in placenta from 25 cases of preeclampsia（PET group,15 women with mild preeclampsia and 10 women with severe preeclampsia） and 20 cases of normal term pregnant women（control group） was detected by semi-quantitative RT-PCR.Results：Expression of UⅡ mRNA in placenta of mild and severe preeclampsia groups were both significantly higher than that in control group（P〈0.05）.Expression of GPR14 mRNA in placenta of severe preeclampsia was significantly higher than that in control group（P〈0.05）.Conclusion：Abnormal expression of UⅡ and its receptor may play a important role in placental ischemia /hypoxia and artherosclerosis.