Caspase-3 mRNA在子痫前期患者胎盘中的表达及其意义

Expression of Caspase-3 mRNA in the placenta of preeclampsia.

目的检测半胱天冬氨酸蛋白酶-3(Caspase-3)mRNA在子痫前期患者胎盘中的表达,以探求Caspase-3在子痫前期发病机制中的意义。方法用半定量逆转录聚合酶链反应(RT-PCR)技术检测正常妊娠(10例),子痫前期(22例)剖宫产后胎盘中Caspase-3基因在转录水平的表达。结果子痫前期组胎盘中Caspase-3 mRNA表达量较正常妊娠组显著升高(P<0.01)。结论Caspase-3在子痫前期的发病中起着重要作用,可能与Caspase-3表达的增加有关。

Objective： Investigating the expression of Caspase-3 mRNA by reverse transcription polymerasechain reaction（RT-PCR） in the normal and preeclampsia placentas in order to evaluate the role of Caspase-3 in preeclampsia.Methods： RT-PCR was used to detect the Caspase-3 expression level in placentas of 22 preeclampsia cases and 10 normal cases after caesarean section delivery.Results： The expression of Caspase-3 was significantly increased in the preeclampsia placentas campared to the normal ones（P〈0.01）.Conclusion：The result suggest that the alteration of Caspase-3 mRNA expression in placenta play an important role in the pathogenesis of preeclampsia.