急性早幼粒细胞白血病TNF-α基因多态性和蛋白表达水平的相关研究

Study on expression levels and gene polymorphisms of TNF-α (tumor necrosis factor alpha) between them in acute promyelocyti leukemia.

目的探讨北方地区汉族人肿瘤坏死因子-α(TNF-α)基因型别的多态性和TNF-α蛋白表达与急性早幼粒细胞白血病(APL)的相关性。方法对21例APL患者采用酶联免疫分析法(ELISA)测定TNF-α含量,应用顺序特异性引物聚合酶链反应(PCR-SSP)方法检测TNF-α基因型别的多态性变化,并与31名健康献血者对照。结果APL组血中TNF-α蛋白含量(1.810±0.116)显著高于对照组(1.107±0.095),P<0.05;APL组和正常对照组TNF-α(308A)等位基因频率分别是(9.524%)和(9.68%)组间差异无统计学意义(P>0.05),提示该等位基因频率与APL不相关,血中TNF-α蛋白表达增高与APL相关。结论中国北方地区汉族人TNF-α基因308位点基因多态性与APL的易感性、临床以及预后可能不起重要作用;而TNF-α蛋白表达与APL的易感性相关联。

Objective：To observe the expression levels and gene polymor-phisms of TNF-α in peripheral blood mononuclear cells of patients with acute promyelocyti leukemia（APL）;to study the effects of the expression levels and gene polymorphisms of TNF-α in the patients with APL.Methods： We select peripheral blood of 21 patients with APL and 31 controls.We use enzyme-linked immunosorbent assay（ELISA） to measure the expression levels of TNF-α in serum;and by using polymerase chain reaction and sequence specific primers（PCR-SSP）,gene polymor-phisms of TNF-α in mononuclear cells are analyzed.Results： The TNF-α levels were higher in APL patients than controls（P〈0.05）;The frequency of TNF-α（308A） genotype was not significantly higher in APL patients than that of controls（P〉0.05）.Conclusion： Our results indicate that the expression levels may be related to development of APL.No significant difference was found in allele frequency at TNF-α（308A） positions.