摘要
本研究采用RAPD技术对来自中国境内8个省市的28群东方蜜蜂Apis cerana进行了研究，探讨了它们之间的亲缘关系。结果表明：15条随机引物共扩增产生了155条谱带，其中单个引物扩增的谱带数为4～16条，多态性条带为122条，多态比率为78．7％；不同种群东方蜜蜂遗传多样性丰富，遗传距离变化较大，最大为0．4197（海南-北京），最小为0．0933（江西-甘肃）；聚类结果显示8个地理种群的东方蜜蜂可聚为3个聚类簇，且各地理种群的亲缘关系与空间距离正相关。
In this study, 28 colonies of Apis cerana from 8 provinces in China were analyzed using RAPD technology and their genetic relationships were discussed. The results showed that a total of 155 dear and reproducible bands were amplified by 15 screened random primers, 4-16 bands per primer and there were 122 polymorphic bands with the polymorphic rote 78.7 %.The genetic diversity was abundant among populations of different regions and the genetic distance varied from 0.4197 （Hainan-Beijing） to 0.0977 （Jiangxi-Gansu）. The dendrogram of eight populations of Apis cerana grouped into three branches, and it showed that the genetic distance of the different populations was positively correlated to the geographic distance.
出处
《动物分类学报》
CSCD
北大核心
2008年第1期89-96,共8页
Acta Zootaxonomica Sinica
基金
山西省留办基金项目(2003051)
山西省教育厅高校科技开发项目(200337)资助
