循环超声法提取万寿菊中叶黄素的研究

Study on Ultrasonic and Circulated Extraction of Xanthophyll from Marigold Flower (Tagetes erecta L)

研究了循环超声技术在万寿菊中叶黄素提取中的应用。实验以万寿菊发酵造粒为原料,对比了不同提取溶剂的提取效果;实验还采用正交试验研究了料液比、超声波功率、超声作用时间对提取率的影响;比较了超声提取与静态溶剂提取法的提取效果。结果显示,最佳提取溶剂为丙酮:石油醚=1:1(V/V);较理想的提取条件为料液比1:70、超声作用时间为20min、超声功率为800W;在此条件下超声循环提取法的提取率为98%,而溶剂静态浸提法仅为42%。

The use of ultrasonic and circulated process was investigated for extraction of xanthophyll from marigold flower. The technical factors of the xanthophylls extraction from marigold flower were researched. The influence of the following factors on the ultrasonic and circulated extraction yield was discussed： selection of solvent, extraction period, ultrasonic power and the ratio between material and solvent. The results showed that 98% of xanthophyll contents could be achieved when optimal ultrasonic and circulated extraction conditions were 1：70 for ratio of material to liquid, 20 min for ultrasonic time and 800 W for ultrasonic power. Compared with ultrasonic and circulated extraction, the normal quiescent solvent extraction could only achieve 42% of xanthophyll contents.