摘要
对富硒菜籽分离蛋白酶水解得到的水解液粗肽产品进行精制,活性炭脱色脱苦,离子交换和透析脱盐处理。脱色工艺在单因素试验的基础上,通过正交试验优化条件为:活性炭用量1.25%,pH3.0,脱色时间60min,温度70℃。两种脱盐方法的脱盐率都很高,均达到85%以上。精制后的产品其平均分子量在1500D以下,是小分子多肽。
Selenium-enriched rapeseed protein peptides were prepared by enzyme-hydrolyzing protein isolates. The crude peptide was refined by discoloring with active carbon and desalination. The single factor tests and orthogonal tests have been applied to optimize the conditions of discolouration as follows： active carbon concentration 1.25%, pH3.0, time 60 min and temperature 70 ℃. The effects of desalination are evident and the desalting efficiency is higher than 85%. The refined peptide produced has lower 1500 D average molecular weight.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第1期129-133,共5页
Food Science
关键词
富硒菜籽蛋白肽
脱色
脱盐
分子量
Selenium-enriched rapeseed protein peptides
discolouration
desalination
molecular weight
