摘要
本实验研究了银耳多糖的提取、分离、纯化及其功能性质。结果表明,酶法浸提效果优于碱浸提法,酶法提取银耳多糖溶液的最适条件为:料水比1:60,加入0.7%的果胶酶,酶解50min,浸提时间60min;银耳多糖通过DEAE-Sepharose Fast Flow和Sephadex G-200柱层析后可得到纯度较高的TPP2多糖。TPP2具有极强的清除DPPH·的活性,DPPH·清除一半时的TPP2浓度为55μg/m(IC50)。
Extraction, isolation, purification and functional properties of Tremella polysaccharides were studied in the paper. The results showed that the enzymatic extraction method was better than the alkali extraction. The optimal conditions for enzymatic extraction are 1：60 of the sample to water ratio, 0.7% of Citrozyme Cloudy, 50 min of enzymatic hydrolysis and 60 min of extraction. A pure polysaccharides TPP2 can be obtained by DEAE-Sepharose Fast Flow and SephadexG-200 purification of Tremella polysaccharides. TPP2 shows the active capability in scavenging DPPH ·. The sample thickness is 55 μg/ml in 50% scavenging rate （IC50）.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第1期134-136,共3页
Food Science
关键词
银耳多糖
提取
酶
纯化
Tremella polysaccharides
extraction
enzyme
purification
