塔里木马鹿单核细胞增多性李氏杆菌的分离与鉴定

Isolation and Identification of Wild Strain of Listeria monocytogenes in Tarim Wapiti

对马鹿流产胎儿的肝脏中的细菌进行分离和鉴定。用亚碲酸钾培养基分离流产胎儿的肝脏中的细菌,并用生化试验、人工感染试验、PCR和测序方法对分离的细菌进行鉴定。该分离株在生化试验、培养特性、溶血性等方面与单核细胞增多性李氏杆菌特性完全相符,能使接种小鼠在24～96h内100%死亡。测序结果表明,扩增P60基因片段与GenBank中报道的单核细胞增多性李氏杆菌强毒株EGD株P60基因核苷酸的同源性分别为99.1%,推导的氨基酸序列的同源性为99.6%。从塔里木马鹿成功分离的细菌为单核细胞增多性李氏杆菌强毒株。

To isolate and identify the bacteria form the liver of aborted fetus of wapiti. The bacteria was isolated in potassium tellurite medium, then identified by biochemistry assay, artifieial infeetion, PCR and sequeneing teehniques. The biologieal eharaeteristie of isolate was identieal with that of Listeria monocytogenes in bioehemistry assay, whieh ean result in 100% fatality in inoeulated miee. The sequeneing results show that the homologies of nueleotide sequenee and dedueed amino acid sequenee between isolate and virulent EGD were 99.1% and 99.6%, respeetively. The strain isolated from fetus of wapiti is a high virulent Listeria monocytogenes .