特油作物蓖麻组织培养关键技术研究初报

Study on Tissue Culture Key Technology on Castor-oil Plant of Special Oil Crop

以蓖麻成熟种子为材料建立无菌系,研究了不同播种方式、不同培养基、不同外植体、不同激素配比对愈伤组织诱导的影响。结果表明:蓖麻种子剥去种皮在以纱布为支持物的培养基上出芽,污染率低,出芽时间提前了27d,幼苗长势也均好于其它培养基;愈伤组织的诱导以叶片为外植体最佳,最适培养基为:MS+2,4D1.0+6-BA0.5+NAA0.02,愈伤组织诱导率达93%,愈伤长势正常。

Aseptic line was obtained from ripe seed of castor-oil plant and the influences of different way of seeded, different basic medium, different explant, different proportion of incretion on callus inducement were studied. The results showed that seeds of castor-oil plant pullulated with lower infection in basic medium supported by gauze. Pullulation was ahead of schedule 27d, and young seedlings growed more better than planted in other basic medium. Leaf used as explant was the best way to induce callus, and substrate ＂MS＋ 2,4D1.0＋6-BA0.5＋NAA0.02＂ was the most appropriate basic medium. The percent of callus inducement reached 93%, and the callus growed normally.