发酵法产氢培养基的响应面分析优化

Statistical Optimization of Process Parameters on Biohydrogen Production from Glucose by Clostridium sp.Fanp2

借助于MINITAB软件,采用Plackett-Burman试验设计法及响应面法分析,对影响高效产氢菌Clostridium sp.Fanp2发酵产氢的培养基成分进行优化研究。首先利用Plackett Burman试验设计筛选出影响产氢的三个主要因素,即葡萄糖浓度、磷酸缓冲液浓度、和维生素液添加量。在此基础上用最陡爬坡路径逼近最大响应区域,再利用Box-Behnken试验设计及响应面分析法进行回归分析。结果表明,葡萄糖浓度、磷酸缓冲液浓度、和维生素液添加量与氢气产量存在显著的相关性,通过求解回归方程得到优化发酵条件:当葡萄糖浓度23.75g/L,磷酸缓冲液浓度0.159M和维生素液添加量13.3ml/L时,氢气产量达到理论最大值4187.7ml/L。经五批培养验证,预测值与验证试验平均值接近,在优化条件下微生物制氢产量提高85.3%。

Statistically based experimental designs were applied to optimizing process parameters for hydrogen production from glucose by Clostridium sp.Fanp2. The important factors influencing hydrogen production, which identified by initial screening method of Plaekett - Burman, were glucose, phosphate buffer and vitamin solution. The path of steepest ascent was undertaken to approach the optimal region of the three sig- nificant factors. Box - Behnken design and response surface analysis were adopted to further investigate the mutual interaction between the variables and identify optimal values that bring maximum hydrogen produc- tion. Experimental results showed that glucose, vitamin solution and phosphate buffer concentration all had significant influence on the specific hydrogen production potential （Ps）. The optimal conditions for the maximal Ps were： glucose 23.75 g/L, phosphate buffer 0.159 M and vitamin solution 13.3 ml/L. After five batches cultivation, the maximum predicted value was consistent with mean value of verification test and the hydro- gen production increased 85.3% at optimized condition.