顽拗植物龙眼果皮中总RNA的提取方法研究

The Method of RNA Isolation from Pericarp of Longan (Dimocarpus Longan Lour.) Fruits

【研究目的】为建立适于富含多糖和多酚等物质的龙眼果皮总RNA提取的方法;【方法】采用TRIZOL法、Tris-硼酸法和改良CTAB法提取龙眼果皮的总RNA,并通过凝胶电泳、紫外分光光度法检测提取的RNA样品的品质;【结果】改良CTAB法、Tris-硼酸法所提取的RNA经电泳检测,28SrRNA和18SrRNA条带清晰,RT-PCR得到的条带与目的片断大小一致,说明所提取的RNA纯度高、质量好;TRIZOL法所得到的RNA降解严重,没有得到完整的电泳条带;【结论】改良CTAB法和Tris-硼酸法适于富含多糖和多酚等物质的龙眼果皮总RNA的提取。

In order to establish a extraction method of total RNA from pericarp of longan Fruits in which polyphenols and polysaccharides are rich, the methods of TRIZOL , Tris - H3BO4 , and improved CTAB was used. The quality of total RNA was analyzed through gel eleetrophoresis and UV spectrometer. The resuits showed that two bands of 28S rRNA and 18S rRNA were appeared after gel eleetrophoresis with the method of improved CTAB and Tris - H3BO4 . The RNA samples were used to amplify eDNA through RT - PCR and the amplifications were observed in agarose gel by eleetrophoresis. The experimental results showed the RNA samples extracted with the method of TRIZOL obtained without gel eleetrophoresis band. The method of improved CTAB and Tris - H3BO4 are suited to extract the RNA of the periearp of longan Fruits.