硫酸铵梯度法制备伊立替康脂质体及稳定性研究

Preparation of Irinotecan Liposome by Ammonium Sulfate Gradient Method and Study on its Stability

目的:制备盐酸伊立替康脂质体并考察提高脂质体稳定性的方法。方法:采用硫酸铵梯度法制备盐酸伊立替康脂质体。以粒径、外观、颜色、渗漏率作为指标,考察盐酸伊立替康脂质体的物理和化学稳定性。结果:硫酸铵梯度法制备的盐酸伊立替康脂质体呈圆球形,平均粒径为600nm,稳定性较好。结论:盐酸伊立替康脂质体不耐高温,将其制成冻干粉剂可显著提高药物稳定性。

Objective： To prepare Irinotecan liposomes and study its stability. Method： Ammonium sulfate gradient method was used to prepare Irinotecan liposomes, size of liposome, appearance, color, oxidation index were observed as indexes, Irinotecan liposome physical and chemical stability was inspected. Result： The average particle size of the liposomes were 600 nm in a pellet shape with a good stability. Conclusion ： Irinotecan liposomes can not bear high temperature. Freeze-dried liposome can obviously improve its stability.