大孔吸附树脂分离纯化资木瓜黄酮的研究

Purification process of flavonoids from Chaenomeles speciosa(sweet) with macroreticular resin

目的:研究大孔吸附树脂分离纯化资木瓜黄酮工艺,为资木瓜黄酮的工业化生产提供依据。方法:采用DM-01,DA-201,DS-401,D-101-Ⅰ,D-101 5种型号大孔吸附树脂对资木瓜黄酮进行吸附纯化,以黄酮收率、纯度为考察指标综合评价,优选出最佳树脂;对优选出的树脂进行洗脱溶剂和洗脱用量的探讨。结果:5种大孔吸附树脂静态饱和吸附量以干树脂计分别为70.80,79.72,66.24,63.12,56.16 mg.g-1(干树脂);静态洗脱率分别为85.76%,79.24%,73.57%,81.45%,71.01%;DM-301型树脂综合性能最好,以50%乙醇为洗脱剂得到的目标产物纯度最高,为96.63%;30%和50%乙醇能洗脱树脂柱上大部分黄酮。结论:DM-301型大孔吸附树脂表现出较好的综合性能,可以用于纯化资木瓜黄酮;用30%和50%的乙醇作为洗脱剂较好。

Objective： To establish a technical process for the purification of flavonoids from Chaenomeles speciosa （sweet） Nakai and provide scientific reference for industrial manufacture. Method： DM-01, DA-201, DS-401, D- 101- I, D-101 types of macroreticular adsorbents were used to separate and purify flavonoids. The adsorption ratio and the elution ratio were compared. Result： The static adsorption capacity of five types of macroreticular adsorbents were 70. 80, 79. 72, 66.24, 63.12, 56. 16 mg · g^-1 （ dried resin） respectively; The static elution ratio were 85.76%, 79. 24%, 73.57%, 81.45%, 71.01% respectively. The purity of flavonoids eluted by 50% ethanol from DM - 301 type of macroreticular was the highest, that is 96. 63%. 30% and 50% ethanol could elute most flavonoids from macroreticular resin. Conclusion： The DM -301 type of macroreticular adsorbents showed better comprehensive adsorption property. It can be used to purify the extract of Chaenomeles speciosa （sweet） Nakai. 30% and 50% ethanol showed better elution property.