摘要
目的:研究葛根黄酮在肠道中的吸收部位和吸收机制。方法:通过对大鼠的十二指肠、空肠、回肠和结肠分别进行在体肠吸收实验,用高效液相色谱法测定葛根黄酮主要成分葛根素的含量,计算出葛根素在各部位的表观吸收速率常数Ka和回流4 h的累计吸收药量,从而反映出葛根黄酮的吸收部位和吸收机制。结果:葛根总黄酮中葛根素在十二指肠、空肠、回肠和结肠的表观吸收速率常数分别为0.013 9,0.012 8,0.012 1,0.013 1 h-1;4h的累计吸收药量分别为136.936,122.739,115.230,123.015μg;其在十二指肠段的吸收明显高于其他肠段(P<0.05)。结论:葛根黄酮在大鼠整个肠道中均有吸收;其吸收呈一级动力学过程。
Objective: To study the mechanism of intestinal absorption of puerariae flavones from various intestinal segments. Method: The intestine in rats was divided into duodenum, jejunum, ileum and colon. The four parts were cannulated in situ recirculation, respectively. With the HPLC, the contain of puerarin which is the major component of puerariae flavones was measured. The apparent absorption rate constant (K.) and the uptake in 4 hours of puerarin in different segments were calculated and compared. Result: The apparent absorption rate constant of puerarin of puerariae flavones at duodenum, jejunum, ileum and colon were O. 013 9, 0.012 8, 0.012 1,0. 013 0 h^-1, respectively, and the uptake of puerarin in 4 hours were 136. 936, 122. 739, 115. 230, 122. 015 μg, respectively. Compared to the other segments, significant higher percentage of puerarin was absorbed from duodenum after being perfused for 4 hours ( P 〈 0. 05 ). Conclusion : The puerariae flavones can be absorbed in whole intestine and the results indicated that the absorption of puerariae flavones compiled with the first order kinetics.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第2期164-168,共5页
China Journal of Chinese Materia Medica
基金
国家高技术研究发展计划(863计划)生物与现代农业技术领域创新药物和中药现代化重大专项(2003AA2Z3249)
关键词
葛根黄酮
在体肠吸收
高效液相色谱法
puerariae flavones
in situ absorption from intestine
HPLC