大孔吸附树脂纯化达原滴丸处方提取液的工艺研究

Purification of Dayuan Dropping Pills extract with macroporous resin

目的研究大孔吸附树脂对达原滴丸提取液的吸附性能,考察精制工艺。方法以达原滴丸中黄芩苷质量分数和收率为主要考察指标,考察了大孔吸附树脂NKA-12、HPD-600、HPD-500、HPD-300、HPD-100、D101、AB-8、NKA-9对达原滴丸中黄芩苷的吸附和解吸特性以及纯化能力。结果达原滴丸提取液上HPD-300树脂,上样液黄芩苷质量浓度为2.65mg/mL,体积流量为3BV/h,50%乙醇解吸5BV时,水洗4BV,解吸效果较好。结论HPD-300树脂可用作达原滴丸的精制。

Objective To study the adsorption function of macroporous resin for purifying the extract of Dayuan Dropping Pills and to investigate the purification process of Dayuan Dropping Pills by macroporous adsorption resin. Methods Taking the content and the extraction rate of baicalin as the main standard index, and the adsorption characters as well as the purification capacities of eight types of macro porous adsorption resins,such as NKA-12, HPD-600, HPD-500, HPD-300, HPD-100, D101, AB-8,and NKA-9, used to treat baicalin of Dayuan Dropping Pills were investigated preliminarily. Results The appropriate adsorption conditions were： the concentration of baicalin was 2. 65 mg/mL, flow rate was 3 BV/ h, the effect of desorption was satisfactory when 50% alcohol （5 BV） and water （4 BV） were used as eluant. Conclusion HPD-300 resin can be applied to refine the Dayuan Droppingp Pills.