何首乌醋酸乙酯提取部位与二苯乙烯苷的调血脂作用

Blood lipid regulation of ethyl acetate extracting fraction and stilbene glycoside from tuber of Polygonum multiflorum

目的研究何首乌醋酸乙酯提取部位(EAFF-PM)与有效成分二苯乙烯苷的调血脂作用。方法观察EAEF-PM和二苯乙烯苷对正常小鼠血脂及肝脏指数的影响;小鼠ipTriton致急性高脂血症模型,观察EAEF-PM和二苯乙烯苷对模型小鼠血脂水平的影响;以大鼠食饵性高脂血症为模型,给予高脂饲料的同时给予EAEF-PM和二苯乙烯苷,连续28d,测定血脂、丙二醛(MDA)、一氧化氮(NO)水平和超氧化物歧化酶(SOD)活性,肝组织中总胆固醇(TC)和甘油三酯(TG)水平以及肝脏指数。结果对正常小鼠,二苯乙烯苷和EAEF-PM均有升高血清高密度脂蛋白胆固醇(HDL-C),降低血清低密度脂蛋白胆固醇(LDL-C)的作用。EAEF-PM和二苯乙烯苷对ipTriton致高脂血症小鼠的血清TC、TG和LDL-C均有降低作用,对HDL-C均有升高作用。EAEF-PM60mg/kg明显降低大鼠食饵性高脂血症模型的肝脏指数;EAEF-PM30、60mg/kg明显降低大鼠食饵性高脂血症模型的血清TC、TG、LDL-C、MDA水平和TC/HDL-C值,明显升高血清HDL-C和NO水平以及SOD活性,二苯乙烯苷对各项指标的作用强度略EAEF-PM,但无显著性差异。结论何首乌中EAEF-PM及二苯乙烯苷均具有调血脂、抗氧化,保护血管内皮的功能,二苯乙烯苷为发挥药效作用的主要物质,提示可用于预防高脂血症。

Objective To investigate the blood lipid regulation of ethyl acetate extracting fraction from the tube of Polygonurn multiflorurn （EAEF-PM） and stilbene glycoside from the tuber of P. rnultiflorurn. Methods The effects of EAEF-PM and stilbene glycoside on blood lipid and liver index were studied in normal mice. Acute hyperlipidemic model mice induced by ip Triton as markers were used to observe the effects of EAEF-PM and stilbene glycoside on the blood lipid level. The investigation was also employed in the hyperlipidemic model rats induced by feeding with the high-lipid diet accompanied by ig EAEF-PM and stilbene glycoside for 28 d, respectively. The effects of EAEF-PM and stilbene glycoside were studied by measuring the concentrations of lipids, malondialdehyde （MDA）, nitric oxide （NO）, and the activity of superoxide dismutase （SOD） in serum of the hyperlipidemic rats. The levels of total cholesterol （TC） and triglyeride （TG） in liver as well as liver index were also simultaneously determined. Results In the normal mice, EAEF-PM and stilbene glycoside not only increased the level of serum high density lipoprotein cholesterol （HDL-C）, also decreased the level of serum low density lipoprotein cholesterol （LDL-C）. EAEF-PM and stilbene glycoside lowered serum TC, TG and LDL-C, and increased serum HDL-C in the hyperlipidemic mice. Administration of EAEF-PM （60 mg/kg） could signi-ficantly reduce the liver index in hyperlipidemic rats. Administration of EAEF-PM （30 and 60 mg/kg） could remarkably decrease the levels of serum TC, TG, LDL-C as well as the ratio of TC/HDL C, also remarkably increase serum HDL- C, NO, and SOD. Compared with EAEF-PM, stilbene glycoside had the somewhat lower effect but no significant difference. Conclusion EAEF-PM and stilbene glycoside possess the obvious effects of blood lipid-regulation, antioxidation and protecting vascular endothelium. The potential constituent is stilbene glycoside which can be used to prevent and/or treat hyperlipidemia.