二氢杨梅素对痤疮丙酸杆菌和脂多糖诱发小鼠肝损伤的保护作用及对中性粒细胞释放白三烯的影响

Protection of dihydromyricetin on liver injured mice induced by Propionibacterium acnes-lipopolysaccharide and its effect on release of leukotrienes from neutrophil

目的研究二氢杨梅素(DMY)对痤疮丙酸杆菌与脂多糖(P.acnes-LPS)诱发小鼠肝损伤的保护作用及对小鼠腹腔中性粒细胞释放白三烯的影响。方法通过对10mg/kgP.acnes负荷5d的小鼠iv3μg/kgLPS建立P.acnes-LPS肝损伤模型,用酶标仪测定血浆丙氨酸氨基转移酶(ALT)活性,RP-HPLC法测定小鼠腹腔中性粒细胞释放白三烯B4(LTB4)和白三烯C4(LTC4)的水平。结果与模型组相比,125、250、500mg/kgDMY均能显著抑制由P.acnes-LPS诱发的小鼠血浆ALT活性升高,其抑制率分别为38.7%、45.1%、49.8%,并对小鼠腹腔中性粒细胞LTB4和LTC4的分泌呈剂量依赖性抑制作用。结论DMY对P.acnes-LPS诱发小鼠肝损伤具有一定的保护作用,其部分作用机制可能与抑制白三烯释放有关。

Objective To observe the protection of dihydromyricetin （DMY） on liver injured mice induced by Propionibacterium acnes-lipopolysaccharide （P. acnes-LPS ） and its effect on release of leukotrienes from murine peritoneal neutrophil. Methods The liver injured model was established by iv 3 μg/kg of LPS into 5 d 10μg/kg P. acnes-primed mice. Hepatic function was evaluated by assessing alanine aminotransferase （ALT） level in plasma with ELISA. HPLC Methods were performed to detect the release levels of leukotriene B4 （LTB4） and leukotriene C4 （LTC4） in murine peritoneal neutrophil. Results Compared with the model groups, 125, 250, and 500 mg/kg DMY inhibited the activities of ALT at 38.7%, 45.1%, and 49.8%, respectively. DMY also inhibited the release of LTB4 and LTC4 in murine peritoneal neutrophil in a dose-dependent manner. Conclusion The results indicate that DMY has the protection to liver injured mice induced by P. acnes-LPS and the mechanisms may be partly related to its inhibitory effect on release of leukotrienes in neutrophil.