摘要
采用PCR-SSP技术检测39份样本的HLA-B27基因并研究甘油在B27基因扩增中的作用,同时与血清学结果比较。7份样本血清学难以判定结果或与PCR结果不一致。PCR技术与血清学方法比较有快速、简便、准确性高等优点。
HLA B27 geng in 39 samples was determined by PCR SSP technique,and the role of glycerine in amplification of B27 gene was studied.The results were compared to serological typing.The results of HLA B27 gene in 7 samples determined by serological genotyping were dubious or not consistent with that obtained by PCR typing.So,PCR method is more rapid,convenient and accurate compared to serological method.
出处
《中国输血杂志》
CAS
CSCD
北大核心
1997年第2期67-68,共2页
Chinese Journal of Blood Transfusion