摘要
目的:应用高效液相色谱法测定复方决明降压胶囊中大黄酚的含量。方法:采用Symmetry C18色谱柱(4.6mm×250mm),以甲醇-0.1%磷酸(85:15)为流动相,流速1mL·min^-1,检测波长254nm。结果:大黄酚的线性范围为0.096-0.480mg,r=0.9999;平均回收率为100.36%(RSD=2.34%)。结论:该方法简便、准确,可作为复方决明降压胶囊的质量控制的依据。
Objective: To set up the determining chrysophanol process in Complex -prescription Capsule of Cassia obtusifolia L to lower blood pressure with high efficiency liquid chromatography. Method: Symmetry C18 chromatographic column (4. 6 mm ×250 mm) was adopted. Methanol - 0. 1% orthophosphoric acid ( 85 : 15 ) had been used as mobile phase, the flow rate was 1 mL · min^-1, and the detecting wave length was 254nm. Result: The linear range of chrysophanol was 0. 096 - 0. 480 mg, r = 0. 9999, and the average recovery was 100.36% (RSD =2. 34% ). Conclusion: The process is convenient and exact, and can be as basis of quality control for Complex - prescription Capsule of Cassia obtusifolia L to lower blood pressure.
出处
《山西中医》
2008年第1期47-49,共3页
Shanxi Journal of Traditional Chinese Medicine
关键词
复方决明降压胶囊
高效液相色谱法
大黄酚
Complex -prescription Capsule of Cassia obtusifolia L to lower blood pressure, high efficiency liquid chromatography, chrysophanol