反义核酸对人白血病HL—60细胞中c—myc基因表达的抑制

INHIBITION OF HUMAN LEUKEMIA c-myc GENE EXPRESSION BY ANTISENSE OLIGODEOXYNUCLEOTIDES

研究两个反义核酸（18mer，21mer）对人白血病HL—60细胞中c－myc基因表达的抑制作用。用HL－60活细胞计数，RNA含量的RT－PCR测定和c－myc基因蛋白的免疫组化染色检查。结果：两个反义寡核苷酸都能抑制HL－60细胞的增殖，增殖抑制率为11％～75％。反义核酸还抑制c－mycmRNA和Myc蛋白的表达，而对c－mcy基因正常表达的红白血病细胞K562的增殖和PHA刺激的人淋巴细胞的增殖没有影响。结论：反义寡核苷酸能抑制c－myc基因的过度表达。

In order to study the effect of two antisense oligodeoxynucleotides (18,21 mer) on the c-myc gene expression.METHODS Numbering living HL-60 cells, Measuring the level of RNA by RT-PCR and staining c-myc gene protein by immunocytochemical method. RESULTS Not only the proliferation of HL-60 cells but also the expression of c-myc gene mRNA and Myc protein were inhibited by antisense oligodeoxynucleotides. Cell inhibition rate ranged in 11%～75%. The inhibition of cell proliferation by antisense oligodeoxynucleotides was sequence-specific, dose-dependent and exposure time-related. Phosphorothioate oligodeoxynucleotides exhibited higher inhibition protency than phosphodiester oligodeoxynucleotides.CONCLUSIONS The antisense oligodeoxynucleotides can inhibite c-myc gene over expression.