期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

小肠上皮细胞分离纯化方法的研究进展 被引量：5

Advance in the Isolation and Culture of Intestine Epithelial Cells
下载PDF
导出
摘要 小肠是消化吸收的重要部位,用获取的小肠上皮细胞来研究小肠功能、营养物质吸收机制等具有重要意义,而成功分离小肠上皮细胞则是前提,可以用组织块培养法、非酶学消化法、酶消化分离法等来分离小肠上皮细胞。本文就目前国内外分离小肠上皮细胞的方法进行了综述。 Small intestine is an important part to absord nutriment. Isolation and culture of intestine epithelial cells is an important means to research mechanism and regulation of nutrition absorption.Tissue culture, nonenzyme digesting and enzyme digesting can be used to isolate the small intestine epithelial cells. The article summarized several means to separate intestine epithelial cells.
作者 王海霞 匡伟 赵国琦
机构地区 扬州大学动物科技学院
出处 《中国畜牧杂志》 CAS 北大核心 2008年第1期54-57,共4页 Chinese Journal of Animal Science
基金 国家自然科学基金(30471259)
关键词 分离方法 成纤维细胞 综述 小肠上皮细胞 isolation methods fibrolast cells a review intestine epithelial cells
分类号 S827.2 [农业科学—畜牧学]
  • 相关文献

参考文献20

  • 1Perreault N, Beaulieu J F. Primary cultures of fully differentiated and pure human intestinal epithelial cells [J]. Exp Cell Res, 1998, 245: 34-42.
  • 2Danes B S, Sutanto E. Epithelial line from normal human colon mucosa[J]. J Natl Cancer Inst, 1982 , 69(6): 1271-1276.
  • 3Kondo Y, Rose I, Young G P, et al. Growth and differentiation of fetal rat small intestinal epithelium in tissue culture. Relationship to fetal age[J]. Exp Cell Res, 1984, 153(1): 121-134.
  • 4Pedersen G, Saermark T, Giese B, et al. A simple method to establish short-term cultures of normal human colonic epithelial cells from endoscopic biopsy specimens. Comparison of isolation methods,assessment of viability and metabolic activity[J]. Scand J Gastroenterol, 2000, 35(7): 772-800.
  • 5Baten A, Sakamoto K, Shamsuddin A M. Long-term culture of nornial human colonic epithelial cells in vitro [J ]. FASET, 1992, 6: 2726-2734.
  • 6廖贤平,李敏.人类小肠上皮细胞原代培养方法探讨[J].武警医学,1998,9(5):251-253. 被引量：15
  • 7Evans G S, Flint N, Somers A S, et al. The development of a method for the preparation of rat intestinal epithelial cell primary cultures[J]. J Cell Sci, 1992, 101: 219-231.
  • 8Foe Llmann W, Weber S, Birkner S. Primary cell cultures of bovine colon epithelium:isolation and cell culture of colonocytes [J]. Toxicol in Vitro, 2000, 14: 435-445.
  • 9Andrea Q, Jack W, Robert L, et al. Epitheliaoid cell cultures from rat small intestine, characterization by morphologic and immunologic Criteria[J]. Cell Biolog, 1979, 80: 248-265.
  • 10张道杰,蒋建新,陈永华,熊健琼,段朝霞,朱佩芳.用嗜热菌蛋白酶进行人肠上皮细胞分离培养[J].第三军医大学学报,2004,26(11):1016-1018. 被引量：17

二级参考文献18

  • 1[1]Stadnyk A W, Dollard C D, Issekutz A C. Neutrophil migration stimulates rat intestinal epithelial cell cytokine expression during helminth infection[J]. J Leukoc Biol, 2000, 68(6): 821 - 827.
  • 2[2]Yu P, Martin C M. Increased gut permeability and bacterial translocation in Pseudomonas pneumonia-induced sepsis[J]. Crit Care Med, 2000, 28(7):2573 - 2577.
  • 3[3]Beaulieu J F, Vachon P H. Reciprocal expression of laminin A-chain isoforms along the crypt-villus axis in the human small intestine[J]. Gastroenterology, 1994, 106(4) :829 - 839.
  • 4[4]Perreault N, Jean F B. Use of the dissociating enzyme thermolysin to generate viable human normal intestinal epithelial cell cultures [J]. Exp Cell Res, 1996, 224(2): 354 - 364.
  • 5[5]Aldhous M C, Shmakov A N, Bode J, et al. Characterization of conditions for the primary culture of human small intestinal epithelial cells [ J ]. Clin Exp Immunol, 2001,125(1):32- 40.
  • 6[6]Hybbinette S, Bostrom M, Lindberg K. Enzymatic dissociation of keratinocytes from human skin biopsies for in vitro cell propagation[J]. Exp Dermatol, 1999, 8(1):30-38.
  • 7[7]Walzer C, Benathan M, Frenk E. Thermolysin treatment: a new method for dermo-epidermal separation[ J]. J Invest Dermatol, 1989, 92( 1 ): 78 - 81.
  • 8[8]Flint N, Cove F L, Evans G S. Heparin stimulates the proliferation of intestinal epithelial cells in primary culture[J]. J Cell Sci, 1994, 107(Pt 2): 401-411.
  • 9Takata K, Hirano H. Changes in soybean agglutinin (SBA) and peanut agglutinin (PNA) binding pattern in the epidermis of the developing chick embryo[J]. Dev Growth Diff, 1983, 25: 299-305.
  • 10De Boeck H, Lis H, van Tilbeurgh H, et al. Binding of simple carbohydrates and some of their chromophoric derivatives to soybean agglutinin as followed by titrimetric procedures and stopped flow kinetics[J]. J Biol Chem, 1984, 259: 7067-7074.

共引文献27

同被引文献59

引证文献5

二级引证文献20

中国畜牧杂志
2008年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部