不同地理株印鼠客蚤酯酶同功酶的比较研究

A Study on Comparison of Esterase Isoenzyme in Different Strain of Xenopsylla cheopis Rothschild

用聚丙烯酰胺凝胶垂直板型电泳的方法，比较分析了印鼠客蚤Ｘｅｎｏｐｓｙｌａｃｈｅｏｐｉｓ大理株和贵阳株不同发育时期的酯酶同功酶酶谱。这两地理株的三龄幼虫、蛹、新羽化成虫和吸血消化７２ｈ后的成虫有酯酶同功酶带１５条，含主带６条，并且它们酯酶带的迁移率和出现频率是基本相同的（Ｐ＞０．０５）。但是，在第４条和第１１条带的显带深浅上，两地理株有差异。

The results of a study on comparison of esterase (Est) isoenzyme in the third instar larvae, pupae, newly emerged adults and sucking adults after 72 h of Dali and Guiyang strain of Xenopsylla cheopis by vertical slab polyacrylamide gel electrophoresis are reported and summarized as follows:1 the Dali and Guiyang strain of X cheopis both showed 15 Est bands with 6 major bands, and the significance test of relative mobility and frequency of thier same bands in different developmental stages had no significance (P>0 05); 2 the 4th Est band of Guiyang strain is more dense than one of Dali strain and the 11th Est band of Guiyang strain is more thin than one of Dali strain