人中性粒细胞防御素对龈下微生物的影响

Effect of Human Neutrophil Defensins on Subgingival Flora

对１８例中、重度成年型牙周病患者的４０颗患牙，在牙周探诊５ｍｍ以上的牙周袋内放置防御素药线一根（含人中性粒细胞防御素８μｇ），４ｄ后取出，实验为期１１ｄ。观察用药前后龈下微生物及临床指标的变化。结果表明，防御素在牙周袋内保持３ｄ后，牙龈指数及出血指数明显改善（Ｐ＜０．０５），龈下菌斑的菌细胞密度降低；同时，球菌的相对比例升高，螺旋体和杆菌的相对比例降低（Ｐ＜０．０５），并能维持到实验结束。本实验结果提示防御素在牙周袋内应用，不仅能降低细菌的数量，还能使龈下菌群的组成向牙周健康部位的菌群组成转化。

teeth of 18 patients with moderate or severe adult periodontitis were examined for evaluating the effect of defensins on subgingival flora. The period of experiment was 11 days. Defensins fibers were put in the periodontal pockets deeper than 5mm for 3 days and discarded on the fourth day. Each of the pockets was applied one fiber containing about 8 μg defensins. The clinical parameters (GI,BI) and the density of subgingival flora were reduced (P<0.05). The increase of coccoid cells, and the decrease of spirochetes and rods were indicated in the relative ratio (P<0.05). This study indicated that defensins could reduce the density of the subgingival flora and change their ratio towards the periodontal healthy flora level.