颊癌中肿瘤转移抑制基因nm23mRNA的表达　Ⅰ.Northern斑点杂交研究

mRNA Expression of Metastasis Suppressor Gene nm23 in Carcinoma of Buccal Mucosa Ⅰ. Northern Blotting Study

通过Ｎｏｒｔｈｅｒｎ斑点杂交技术研究５２例颊癌及相关组织中肿瘤转移抑制基因ｎｍ２３－Ｈ１和ｎｍ２３－Ｈ２ｍＲＮＡ的表达。结果发现：颊癌组织中ｎｍ２３－Ｈ１和ｎｍ２３－Ｈ２ｍＲＮＡ表达比正常颊粘膜、白斑、癌旁粘膜均有不同程度增高。颊癌有转移组中ｎｍ２３－Ｈ１ｍＲＮＡ表达比无转移组明显降低，转移灶中ｎｍ２３－Ｈ１ｍＲＮＡ的表达更低（Ｐ＜０．０５）。１１例有转移颊癌患者中有９例（８１．８％）为ｎｍ２３－Ｈ１低表达；而１９例无转移颊癌，有１５例（７８．９％）为高表达（Ｐ＜０．０５）。ｎｍ２３－Ｈ２ｍＲＮＡ表达在有无转移组患者中无明显差异（Ｐ＞０．０５）。结果提示，在颊癌的转移过程中，ｎｍ２３－Ｈ１比ｎｍ２３－Ｈ２起着更重要的作用。ｎｍ２３－Ｈ１ｍＲＮＡ表达可作为预测有无颊癌淋巴结转移的一个有价值的指标。

In this study the authors detected both nm23 H 1 and nm23 H 2 mRNA levels in 52 tissues samples of patients with carcinoma of buccal mucosa (CBM) by Northern blotting method. The expression levels of both nm23 H 1 and nm23 H 2 mRNA in CBM were higher than those in normal buccal mucosa, leukoplakia, adjacent nontumorous mucosa, and lymphonodes with or without metastasis. The nm23 H 1 mRNA expression levels in CBM with lymphonodes metastases were lower than those in CBM without metastasis, and were lower in metastatic lymphonodes (P<0.05). Of the 11 cases with metastases, 9 cases (81.8%) showed low expression of nm23 H 1 mRNA, of 19 cases without metastasis, 15 cases (78.9%) were high expression. No significance of nm23 H 2 mRNA expression levels were found between CBM with and CBM without metastasis (P>0.05). The expression of nm23 H 1 and nm23 H 2 mRNA showed no significant correlation with each clinical pathological parameter examed. The result suggested that the expression of nm23 H 1 mRNA significantly correlated inversely with lymphonode metastasis in CBM, while the expression of nm23 H 2 mRNA not. nm23 mRNA expression levels can provide a valuable predictor of lymphonode metastases in CBM.