相对的两对引物-聚合酶链反应技术在乙醛脱氢酶-2基因分型中的应用

Application of polymerase chain reaction with confronting two-pair primers in aldehyde dehydrogenase-2 typing

目的了解乙醛脱氢酶-2（ALDI-12）基因型在广西壮族和汉族健康人群中的分布及其对饮酒行为的影响。方法分别采用相对的两对引物．聚合酶链反应（PCR-CTPP）和聚合酶链反应-限制性片段长度多态性方法（PCR—RFLP）检测193名汉族和88名壮族成人ALDI-12基因型，问卷调查其饮酒行为。结果壮族和汉族人群ALDH2^2基因频率分别为0．511、0．489，ALDH2^2基因频率分别为0．508、0．492，差异无统计学意义（x^2=0．001，P〉0．05）。PCR—CTPP的分型结果与PCR—RFLP结果相符。高频饮酒且携带ALDI-12。纯合基因型者在汉族和壮族各占15．67％和35．59％，差异有统计学意义（X^2=5．800，P=0．016）。结论广西汉族和壮族人群中ALDH2基因型频率分布无差异，不同民族人群的ALDH2遗传型可能对其饮酒行为有影响。

Objective To study the distribution of aldehyde dehydrogenase-2 （ALDH2） polymorphisms between healthy Zhuang and Han ethnic individuals in Guangxi Autonomous Region and its influence to the behaviors of alcohol consumption. Methods Polymerase chain reaction with confronting two-pair primers （PCR-CTPP） and polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） techniques were used to genotype ALDH2, respectively, and alcohol consumption was recorded in a constructed questionnaire. Results The frequencies of ALDH2 alleles （ALDH21/ALDH22） among Zhuang and Han ethnics were 0. 511,0. 489 and 0. 508,0. 492 respectively （ X^2 = 0. 001, P 〉 0. 05 ）. The ALDH2 genotypes were verified with PCR-RFLP method. The frequencies of ALDH21 genotype in alcoholics （ ≥3 times drinking per week） were 35.59% and 15.67% in Zhuang and Han groups respectively（ X^2 = 5. 800, P = 0. 016）. Conclusion There was no significant different distribution of ALDH2 genotype among healthy Zhuang and Han ethnic people. The genotype of ALDH2 in different ethnicity might influence individual behavior of alcohol consumption.