摘要
目的建立反相高效液相色谱法测定宁夏枸杞中绿原酸含量的方法。方法色谱分析条件Shim-pack VP-ODS色谱柱（250mm×4.6mm,5μm）,以乙腈∶水∶冰乙酸（5∶94.5∶0.5）为流动相A,乙腈∶水∶冰乙酸（70∶29.5∶0.5）为流动相B,采用梯度洗脱,流速0.5ml/min,检测波长342nm,柱温30℃。结果绿原酸线性范围为10～100μg/ml（相关系数为r=0.9995）,平均回收率为98.6%（n=5）;不同产地、不同年份宁夏枸杞样品中绿原酸含量存在差异,随保存时间的延长,绿原酸含量减少,但减少程度不明显。结论本方法重现性好,适用于测定枸杞中绿原酸的含量。
Objective To develop a RP-HPLC method with gradient dilution for determination of chlorogenic acid in Lycium barbarum.L from different habitats.Methods The separation was performed on a Shim-pack VP-ODS 250 mm ×4.6 mm（5 μm）,acetonitrile/water/acetic acid（5 ∶ 94.5 ∶ 0.5）was used as the mobile phase A and acetonitrile/water/acetic acid（70 ∶ 29.5 ∶ 0.5）as the mobile phase B with gradient elution,the flow rate was 0.5 ml/min,the detective wavelength was at 342 nm and the temperature was 30℃.Results The good linearity of chlorogenic acid was obtained over the ranges of 10~100 μg/ml（r=0.999 8）,the average recovery was 98.6%（n=5）; the content of chlorogenic acid in Lycium barbarum.L from different habitats was different.Conclusion Being rapid and reproducible,the method can be used to determine chlorogenic acid in Lycium barbarum.L.from different habitats.
出处
《中国现代药物应用》
2008年第1期51-53,共3页
Chinese Journal of Modern Drug Application
