补锌对冷暴露大鼠肝亚线粒体H^+转运ATP酶的影响

EFFECTS OF HIGH-ZINC INTAKE ON H +-TRANSPORTING ATPASE IN HEPATOCELLULAR MITOCHONDRIA IN COLD EXPOSED RATS

采用ＳＤ雄性大鼠３６只，随机分为三组，分别为正常饲料常温对照组，正常饲料冷暴露组和高锌饲料冷暴露组，１４ｄ后宰杀动物，收集样品。冷暴露１４ｄ后，正常饲料冷暴露组大鼠肝及骨骼肌中锌有降低趋势，肝亚线粒体Ｈ＋转运ＡＴＰ酶活性及其对寡霉素的敏感性均明显降低，膜的流动性降低（Ｐ＜０．０５）；高锌饲料组大鼠肝及骨骼肌中锌均明显升高（Ｐ＜０．０５）。肝亚线粒体Ｈ＋转运ＡＴＰ酶活性及其对寡霉素的敏感性虽低于对照组，但较正常饲料冷暴露组均明显升高，膜的流动性也明显升高（Ｐ＜０．０５）。补锌可以提高冷暴露大鼠体内的锌水平，高锌对冷暴露造成的膜损伤有防护作用，维持Ｈ＋转运ＡＴＰ酶的构象，提高该酶的活力。

Thirty-six male SD rats were randomized into 3 groups. Rats from group 1, 2 and 3 were treated in the temperature of 20±1℃ with common ration(50μg/kg), treated in the temperature of 1±1℃ with common ration and treated in the temperature of 1±1℃ with high-zinc ration(100μg/kg), respectively. 15 days later, rats were slaughtered for detection of the levels of Zn in serum, liver, and skeletal muscle, the membrane fluidity, the activity of H +transporting ATPase, and its sensitivity to oligomycin of submitochondria in hepatocytes.The findings revealed that the concentrations of Zn in the liver and skeletal muscle of group 3 were higher than those of the group 1 and 2(P<0.05).The membrane fluidity, the activity of H +transporting ATPase and its sensitivity to oligomycin in group 3 were significantly greater than those of the group 2. The findings indicated that supplement with zinc might increase the level of zinc in cold-exposed rats. High-zinc intake might protect the membrane from injury by cold exposure, maintain the structure of H +-transporting ATPase and boost its activity