聚磷酸钙骨支架材料的可控降解性和细胞毒性研究

Controllable Degradation and Cytotoxicity of Calcium Polyphosphate Bone Scaffold

采用重力二次烧结法制备了聚磷酸钙(CPP)骨支架材料,并对材料的体外可控降解性和细胞毒性进行了研究。实验结果表明,CPP呈线性链状结构,具有无定形态、γ-CPP和β-CPP3种结构。晶相对CPP的降解速率影响明显,无定形CPP降解最快,10d完全降解;β-CPP降解最慢,30d约失重11%。同时,材料的降解速率随烧料粒径的增大而加快。细胞在材料表面粘附铺展且增殖良好。制备的CPP骨支架材料具有优良的可控降解性和生物相容性,可用于修复骨组织缺损和作为支架材料用于组织工程。

Calcium polyphosphate（CPP）bone scaffold was prepared by gravity sintering.The cell culture with CPP and the controllable biodegradation of CPP in vitro were investigated.The results revea1 that calcium polyphosphate is a long chain linear inorganic condensed phosphate with amorphous CPP、γ-CPP and β-CPP three different structures.The degradation behavior of CPP is obviously affected by crystalline structure.Amorphous CPP is the sample of the fastest degradation rate,which is completely degraded in 10 d.After immersion in SBF for 30 d,the weight loss of γ-CPP and β-CPP is 35% and 11%,respectively.In addition,the biodegradation ratio quickens with the increasing of CPP particle size.The cultural cells are attached,dispersed and preferentially proliferated on the surface of scaffolds.CPPs not only have good biocompatibility but also possess controllable biodegradation.This type of CPP biomaterial can be used as scaffolds for bone defect repair and tissue engineering.