微滴度板杂交试验在疟疾种属特异性诊断的应用

Application of Microtiter Plate-Hybridization Methods for the Detection of Malaria Parasites in the Blood Sampled on Filter-Paper Disks in Solomon Islands

本实验应用113份指尖取血制作的滤纸片干血滴样品分离疟原虫DNA进行微滴度板杂交试验。结果表明：1．滤纸片干血滴一微滴度板杂交实验用血量少、经过自然干燥后，样品易于保存和运输，而且提取DNA的方法简便。2．此方法特异性高、敏感性强、其检测极限可达到1．5个原虫／μl血。3．此方法可在进行疟疾诊断的同时进行种属鉴定，并可对多种疟原虫同时感染进行诊断。

113 samples of blood collected by finger-prick of the inhabitants in Solomon islands on filter paper disks were examined with microtiter plate-hybridization method. The result suggested:1). Only 5 μl blood was used in this experithent and after natural dry,it was easy to store and to transport the blood sampled on filter-paper disks. The isolation of DNA from the filter paper was very simple. 2). This method has high specificity,.sensitivity and the detection limit of this method can come up to 1. 5 worm/ul blood. 3). This method can be used not onlyfor diagnosis of malaria infection with single species and identification of the species of malaria,but for diagnosis of the concurrent infection with multiply species of malaria.