摘要
本文采用酶联免疫电转移印迹技术（EITB）分析、比较日本血吸虫不同发育时期虫体特定的组分蛋白分子、雌虫、雄虫和虫卵抗原分别与相应的雌、雄虫和虫卵免疫血清反应呈现17、20和8条蛋白带，这三种抗原与其它不同时期虫体免疫血清反应，三者间及彼此间均出现交叉反应，而雌、雄虫和虫卵抗原与其它寄生虫感染血清作用没发现有叉及反应。应用间接荧光抗体试验（IFA）和免疫酶染色试验（IES）对日本血吸虫抗原进行定位研究，其结果相似。血吸虫主要抗原物质来源于成虫表皮、肠上皮和卵内的毛蚴.
This paper dealed with specific proteins in different developing stages of Schistosonza japonicum using enzyme linked immunoelectrotransfer blot (EITB). Antigens of female. male and egg reacted with antisera to the three antigens respectively showed 17. 20 and 8 distinct protein bands. These antigens appeared cross-reactions with antisera in the different stages. But,they had not cross-reaction with sera of infected other parasites. In IFA and IES the results of antigen location were similar The antigenic proteins mainly presented on the tegumental surface and the epithelial cell of adult worm gut and miracidium in egg.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1997年第5期31-35,共5页
Chinese Journal of Zoonoses
基金
世界卫生组织热带病研究与培训专门规划司的资助
