应用基质辅助激光解吸电离飞行时间质谱检测细菌耐药性的初步研究

Primary research on detecting bacteria drug resistance by matrix-assisted laxer desorption ionization time-of-flight mass spectrometry

目的研究基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)用于检测鉴定细菌耐药性的可行性。方法对临床分离的耐药谱明确的8属11株细菌及相应的标准菌株,经相同条件培养后,应用MALDI-TOF-MS进行检测,并分析其耐药株和标准株质谱图的差异。另外,对临床分离的30株金黄色葡萄球菌同时进行药物敏感性实验和MALDI-TOF-MS检测。结果应用MALDI-TOF-MS检测细菌耐药性时,对葡萄球菌检测结果较好,尤其是耐甲氧西林金黄色葡萄球菌,而对其它细菌耐药性检测效果较差。结论MALDI-TOF-MS检测细菌耐药性适用于部分细菌。

Objective To study the feasibility of bacteria drug resistance determinated by matrix-assisted laxer desorption ionization time-of-flight mass spectrometry（MALDI-TOF-MS）. Methods 11 strains of 8 genera in drug resistant-bacteria separated from clinical specimens and the corresponding standard strains were detected by MALDI-TOF- MS, and the differences of the mass spectrums were analyzed. At the same time, 30 strains of Staphylococcus aureus separated from clinical specimens were detected by MALDI-TOF-MS and their antimicrobial susceptibility were tested. All bacteria were cultured under the same conditions. Results When drug-resistant bacteria determinated by MALDI-TOF- MS, it was efficient for Staphylococcus, especially the methicillin-resistant Staphylococcus aureus, but it was not efficient for other genera. Conclusion MALDI-TOF-MS can be used to detect drug resistance of some bacteria.