缺氧诱导因子-lα和血管内皮生长因子在人脑胶质瘤中的表达及意义

Expression ofHIF-1α and VEGF in human glioma and their significance in the pathogenesis thereof

目的:探讨缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)和微血管密度(MVD)在人脑胶质瘤中的表达情况,分析三者的关系及其意义。方法:应用免疫组化方法分析62例人脑胶质瘤中HIF-1α、VEGF、和MVD的表达情况。结果:本组HIF-1α的总阳性表达率是66.1%,它们在正常脑组织和Ⅰ-Ⅱ、Ⅲ、Ⅳ肿瘤组织的阳性率分别为0%、39.3%、81.8%、100%与正常对照组比较有显著性差异(P<0.01)。HIF-1α表达水平与胶质瘤恶性度存在相关性(P<0.01)。VEGF在HIF-1α阳性组中的阳性率(95.1%)明显高于HIF-1α阴性组中的VEGF的阳性率47.6%(P<0.01)。结论:HIF-1α和VEGF的表达与MVD存在正相关关系;VEGF与HIF-1α表达呈正相关性,二者与人脑胶质瘤的病理分级呈正相关,与脑胶质瘤新生血管生成有关。

Objective： To investigate the expression of the hypoxia-inducible factor（ HIF-1α） and vascular endothelial growth factor（VEGF） in human golimas and to analyze the realation among them. Methods： Expression of the HIF-1α and VEGF was investigated by immunohistochemistry in 62 specimens of human gliomas. The microvessel density （MVD） was observed by microscopy. Result： the HIF-1α positive rate was 66. 1% ,s The level of HIF-1αwas 39.3% in glioma grade Ⅰ -Ⅱ ,81.8 % in glioma grade Ⅲ, 100 % in glioma grade Ⅳ. The expression of HIF-1αwas positively correlated with the glioma grades, their statistical significance in groups （P〈0.01） . the expression of HIF-1α was correlated with staging of the human gliomas （P〈0.01）. The VEGF positive rate of the HIF-1α-positive group was 95.1%, significance higher than that of the HIF-1α-negative group（47.6 %） ,P〈0.01. the MVD strongly positively correlated with the HIF-1α and VEGF. Conclusion： VEGF upregulate the HIF-1α expression. Both of them was upregulate with the staging of the human gliomas and correlated with the novel ngiogenesis of the human golimas.