基质金属蛋白酶-9基因多态与急性脑梗死

Relationship between matrix metalloproteinase-9 polymorphism and acute cerebral infarction

目的探讨基质金属蛋白酶-9（matrix metalloproteinase-9，MMP-9）基因多态及血清水平与急性脑梗死的关系。方法 对2006年1月至2007年5月期间在我院神经内科住院的急性脑梗死患者101例和我院体检中心健康体检者114名进行研究，采用ELISA法测定血清MMP-9水平，同时采用聚合酶链反应．限制性片段长度多态性（PCR-RFLP）方法分析MMP-9基因启动子C-1562T多态。结果脑梗死患者组48h内血清MMP-9水平为（138．9±121．8）ng／ml，而对照组为（18．4±4．6）n∥Inl，两组差异有统计学意义（t=9．93，P=0．00）。脑梗死组CT基因型患者外周血MMP-9水平为（113．3±66．6）ng／ml。而cc基因型患者外周血MMP-9水平为（148．6±104．9）ng／ml，两者相比差异无统计学意义（t=1．22，P=0．21）。脑梗死组CT＋TT基因型频率13．9％，对照组为13．2％，两者比较差异无统计学意义（X^2=0．02，P=0．88），T等位基因频率在脑梗死组为6．9％，对照组为7．5％，两者比较差异也无统计学意义（r=0．04，P=0．83）。结论MMP-9基因启动子-1562位点的多态与MMP-9基因表达和脑梗死没有明确的关系。MMP-9基因启动子C-1562T的多态与缺血性脑血管疾病的关系有待进一步研究。

Objective To investigate the relationship between acute cerebral infarction （CI） and matrix metalloproteinase-9 （MMP-9） serum level and polymorphism （C - 1562T） in Hart population. Methods One hundred and one patients with acute CI from the department of neurology of Taizhou Hospital were included and 114 healthy persons were selected from physical examination as the control group. Serum MMP-9 level was measured by enzyme-linked immunosorbent assay （ELISA）. At the same time, genotype was determined by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） for the common C - 1562T functional promoter polymorphism of the MMP-9 gene. The serum MMP-9 level and genotype frequencies of the MMP-9 gene between the patients and the control group were analyzed. Results The genotype frequencies of the MMP-9 gene C - 1562T polymorphism of the two groups were in Hardy- Weinberg equilibrium. The results of individual polymorphisms analysis showed that the frequencies of CT and ＂TT＂ genotypes in the C - 1562T polyporphismin were of no significant difference between the patients group with CI （ 13.9% ） and the control group （ 13. 2% ）. The frequencies of - 1562T allele was of no statistical difference between the CI group （ 6.9% ） and the control group （ 7.5% ）. But serum levels of MMP-9 in CI patients group （（ 138.9 ± 121.8 ） ng/ml） were significantly higher than in the control group （（18.4±4.6） ng/ml, t=9.93, P=0.00）. Conclusions Serum level of MMP-9 obviously is increased after ischemic stroke in 48 hours. But genetic polymorphism in MMP-9 promoter（C - 1562T） has no definite relationship with MMP-9 genetic expression and CI in the Hart population of China. Therefore, the relationship between genetic polymorphism in MMP-9 promoter（ C -1562T） and ischemic stroke needs further investigation.