白细胞介素-6mRNA及其蛋白在烧伤复合内毒素血症早期大鼠肝组织中的原位表达

Expression of interleukin 6 mRNA and its protein in the liver at the early stage after burns complicated with endotoxemia in rats

目的：观察ＩＬ－６ｍＲＮＡ及其蛋白在烧伤复合内毒素血症早期大鼠肝损害中的表达和细胞定位以及探讨ＩＬ－６在其发病过程中的作用。方法：选用１５６只Ｗｉｓｔａｒ大鼠随机分为复合组（Ｃ）、烧伤对照组（Ｂ）、内毒素对照组（Ｅ）和正常对照组（Ｎ）。应用血清定量、免疫组化（ＩＨＣ）及原位杂交（ＩＳＨ）等方法，检测了烧伤复合内毒素血症大鼠血清ＩＬ－６含量变化、肝组织ＩＬ－６和ＩＬ－６ｍＲＮＡ的细胞定位及其分布。结果：血清ＩＬ－６分别在０．５ｈ、６～１２ｈ出现两个峰值（Ｐ＜０．０５），组织ＩＬ－６主要定位于肝窦内皮细胞（ＳＥＣｓ）、枯否细胞（ＫＣｓ），ＩＬ－６ｍＲＮＡ主要定位于ＫＣｓ、中性粒细胞（ＰＭＮｓ）、巨噬细胞（ＭＰｓ）。结论：提示ＩＬ－６是参与烧伤复合内毒素血症早期肝脏损害的重要细胞因子之一，并与肝细胞ＬＢＰ的合成密切相关。

Objective: To observe the distribution and cell localization of interleukin 6 (IL 6) mRNA in the liver after burns complicated with endotoxemia. Methods: After 156 Wistar rats were randomized into complex, burn, endotoxin intoxicated and normal control groups, the changes of the content serum IL 6 and the expression and localization of liver IL 6 and IL 6 mRNA were investigated with serum quantification, immunohistochemistry and in situ hybridization. Results: Serum IL 6 reached its peak for twice at the 0.5th and 6th(12th h respectively. IL 6 mainly localized in sinusoid endothelial cells and Kupffer cells (KCs) while IL 6 mRNA in KCs, polymorphonuclear neutrophils and macrophages. Conclusion: IL 6 is one of the key cytokines in liver damage after burns complicated with endotoxemia in rats and closely correlated to the synthesis of lipopolysaccharide binding protein in hepatocytes.