桑天牛成虫交配后血淋巴和生殖系统内可溶性总糖和蛋白质含量的变化

Effect of mating behavior on the total carbohydrate and protein content of Apriona germari Hope adults

以生理状况相似的未交配桑天牛Apriona germari Hope雌、雄成虫为供试昆虫,观察、记录交配活动;采集血淋巴,解剖生殖系统并绘图;用蒽酮比色法和福林-酚法检测交配前、后成虫血淋巴和生殖系统内可溶性总糖和蛋白质含量变化。结果表明:交配后1h,桑天牛雄虫血淋巴内的可溶性总糖含量增加21.38%,蛋白质含量降低22.66%;雄虫生殖系统内的可溶性总糖和蛋白质含量都明显升高;雄性附腺作为某些特异性蛋白质的合成场所其内的可溶性总糖和蛋白质含量分别降低81.76%和63.76%,雌虫血淋巴和卵巢内的可溶性总糖和蛋白质含量都升高。交配后,雄虫发生陪伴行为最短历时为4h,可能是其重要的生殖策略之一。

The mating behavior between the female and male of Apriona germari Hope with the similar physiological status was observed. The reproductive systems of both sexes, including male reproductive system, male accessory gland and ovary, were isolated and described. The contents of the total carbohydrate and protein in reproductive systems and the hemolymph from both virgin and mated male and female were also tested by anthrone colorimetry and Folin-phenol methods. The results showed that in males one hour after mating, the contents of the total carbohydrate and protein in the reproductive system both increased, the content of protein in the hemolymph decreased by 21.38%, and the contents of the total carbohydrate and protein decreased by 81.76 % and 63.76 %, respectively, in the male accessory glands which secreted some special proteins used as the mating factors. As for females, the total carbohydrate and protein in haemolymph and ovary both increased during the whole mating process. The company behavior of the male, which would be an important part of its reproductive strategies, would last over 4 h.